Testing on SuperCycler performance (Biometra)

• SuperCycler test result compared with Biometra.

Consistency:


1. Amplification of M13 MCS region of pTZ57R at Ta of 52°C on 12 tubes which placed at random location of the 96-well block of SuperCycler. The same PCR reaction mixture was amplified concurrently at 3 units of Biometra Tpersonal PCR machine as control.

Result :

Legend:
M: VC1kb DNA Ladder
Lane 1-12: Amplification on Kyratec SuperCycler
Lane B1-B3: Amplification on Biometra Tpersonal


Gradient PCR:

1. Amplification of M13 MCS region of pTZ57R (1.5kb) on 12 tubes which placed in a row of the 96-well block of SuperCycler. The same PCR reaction mixture was amplified concurrently at 3 units of Biometra Tpersonal PCR machine at different annealing temperature as control.

Result:

Legend:
M: VC 1kb DNA Ladder
Lane 1: Ta at 45°C          Lane 7: Ta at 55.9°C
Lane 2: Ta at 46.8°C       Lane 8: Ta at 57.7°C
Lane B1: Ta at 46.8°C     Lane 9: Ta at 59.6°C
Lane 3: Ta at 48.6°C       Lane 10: Ta at 61.4°C
Lane 4: Ta at 50.4°C       Lane B3: Ta at 61.4°C
Lane 5: Ta at 52.3°C       Lane 11: Ta at 63.2°C
Lane 6: Ta at 54.1°C       Lane 12: Ta at 65°C
Lane B2: Ta at 54.1°C

2. Amplification of plant chloroplast DNA region of plant DNA (1.5kb) on 12 tubes which placed in a row of the 96-well block of SuperCycler. The same PCR reaction mixture was amplified concurrently at 3 units of Biometra Tpersonal PCR machine at different annealing temperature as control.

Result :

Legend:
M: VC 1kb DNA Ladder
Lane 1: Ta at 45°C             Lane 7: Ta at 55.9°C
Lane 2: Ta at 46.8°C          Lane 8: Ta at 57.7°C
Lane B1: Ta at 46.8°C       Lane 9: Ta at 59.6°C
Lane 3: Ta at 48.6°C          Lane 10: Ta at 61.4°C
Lane 4: Ta at 50.4°C          Lane B3: Ta at 61.4°C
Lane 5: Ta at 52.3°C          Lane 11: Ta at 63.2°C
Lane 6: Ta at 54.1°C          Lane 12: Ta at 65°C
Lane B2: Ta at 54.1°C

3. Amplification of plant chloroplast DNA region of plant DNA (360bp) on 12 tubes which placed in a row of the 96-well block of SuperCycler. The same PCR reaction mixture was amplified concurrently at 3 units of Biometra Tpersonal PCR machine at different annealing temperature as control.

Result:

Legend :
M: VC 1kb DNA Ladder
Lane 1: Ta at 45°C               Lane 7: Ta at 55.9°C
Lane 2: Ta at 46.8°C            Lane 8: Ta at 57.7°C
Lane B1: Ta at 46.8°C         Lane 9: Ta at 59.6°C
Lane 3: Ta at 48.6°C            Lane 10: Ta at 61.4°C
Lane 4: Ta at 50.4°C            Lane B3: Ta at 61.4°C
Lane 5: Ta at 52.3°C            Lane 11: Ta at 63.2°C
Lane 6: Ta at 54.1°C            Lane 12: Ta at 65°C
Lane B2: Ta at 54.1°C

Conclusion: There is no significant difference in the quality of results between the Kyratec SuperCycler and the Biometra Tpersonal machines.